S1P5 is required for NK-cell trafficking to BM and LN sinusoids. Flow cytometry analysis of BM and LN NK cells isolated from WT and S1P5^−/− mice injected with anti-CD45 and then stained in vitro with Abs for CD19, CD3, NK1.1, CD27, and CD11b. The percentage of CD45⁺ cells among gated NK cells (A), NK-cell subsets (B), or B and T cells (C) was measured in WT (black bars) and S1P5^−/− mice (white bars). Data show the mean ± SD of 5-10 mice in each group.