NK cells accumulate in the BM and LNs of Cxcr4^+/1013 mice. Flow cytometric analysis of NK cells in the blood, spleen, LNs, and BM from WT and Cxcr4^+/1013 mice stained for CD3 and NK1.1 expression. (A) Representative dot plots of CD3/NK1.1 expression. (B-C) Mean frequency (B) and number of gated NK cells (C; NK1.1⁺ CD3⁻) in the different organs. (D) Flow cytometric analysis of NK cells in the BM and LNs from WT and Cxcr4^+/1013 mice injected with anti-CD45 mAb and then stained in vitro with Abs for CD3 and NK1.1. The percentage of CD45⁺ cells among gated NK cells was measured. Results represent the means ± SD of 7-8 mice in each group (B) with 1 dot-plot representing 1 mouse (A), all analyzed littermates (C, lines indicate the mean and each symbol represents an individual mouse), or are from 4 independent experiments (D).