Quantitative RT-PCR assay. (A) Quantitative RT-PCR assay for GEP. (B) Quantitative RT-PCR study for miRNAs. Expression data (RQ mean) of the validated quantitative RT-PCR genes and miRNAs included in the NMZL signature compared with RLN and FL. RQ values are shown below the corresponding graph, and the t test (P) values are shown on the bars for each comparison. The genes and miRNAs that appear in Tables 2 and 4 (and that are not shown in Figure 5) had no valuable data in the quantitative RT-PCR assay (Ct values > 36): IKIP gene and miR-34a, miR-625, miR-222, miR-202, miR-765, miR-370, and miR-513. Oblique striped represent RLN cases; black, NMZL cases; and light gray, FL cases.