eGFP-c-Myc protein expression is induced by polyI:C and IFN-α in HSCs. (A) Treatment of eGFP-c-Myc knock-in mice with polyI:C induces eGFP-c-Myc expression in HSCs as shown by flow cytometry. Sca-1 was omitted as a marker, as polyI:C leads to a shift in Sca-1 expression. (B) Quantification of A (pooled data of 3 independent experiments; N = 12; mean and SD; ****P < .001; unpaired 2-tailed t test). (C) Correlation of cell cycle status (Ki67-Hoechst) and eGFP-c-Myc expression of HSCs after polyI:C treatment (N = 3; mean and SD; *P < .05; unpaired 2-tailed t test). (D) mRNA expression of c-Myc and N-Myc were measured by qRT-PCR in FACS-sorted Lin⁻Kit⁺CD48⁻CD150⁺ cells of wild-type mice injected with phosphate-buffered saline or polyI:C (pooled data of 2 independent experiments; N = 6-8 per group; mean and SD; unpaired 2-tailed t test).