Gene expression analysis in platelets of transgenic mice and controls by quantitative PCR. Fold changes in mRNA expression of selected genes in platelets of SclCre^ER;V617F;Stat1^−/− and controls (A), and VavCre;V617F;Stat1^−/− and controls mice (B). Expression of mouse Gusb was used for normalization and relative expression was calculated with the ΔCT method. The mean value of 4 mice per group is shown. Error bars represent SEM. One-way ANOVA is shown for comparisons. (C) Serum levels of IFNγ in transgenic mice and controls. Duplicates of 1 mouse per genotype are shown. (D) Serum levels of IFNγ in patients with JAK2-V617F–positive and JAK2 exon 12–mutated MPN, and healthy controls. The Student t test is shown for the comparisons. *P ≤ .05, **P ≤ .01. NC, normal control; ns, not significant; PCR, polymerase chain reaction; wt, wild type.