American Society of Hematology

Figure 1

$Figure 1. Neutrophils rolling on P-selectin transition to integrin-dependent arrest in trauma-stimulated venules of EHCgp130−/− mice. Neutrophil rolling flux fraction (A), rolling velocity (B), and firm adhesion (C-D) were measured in venules of cremaster muscle from WT and EHCgp130−/− mice subjected to surgical trauma to mobilize P-selectin and CXCL1 to the venular surface. Before surgery, some mice were injected intravenously with blocking mAbs to P-selectin (P-sel), β2 integrins (β2), CXCL1, or CXCR2, or with PTx. Basal neutrophil rolling flux fraction (E) and rolling velocity (F) were measured in dermal venules of ear in the absence of trauma. The data represent the mean ± SEM from 15 to 20 venules from 4 to 5 mice in each group. *P < .05, **P < .01.$

Neutrophils rolling on P-selectin transition to integrin-dependent arrest in trauma-stimulated venules of EHCgp130^−/−mice. Neutrophil rolling flux fraction (A), rolling velocity (B), and firm adhesion (C-D) were measured in venules of cremaster muscle from WT and EHCgp130^−/− mice subjected to surgical trauma to mobilize P-selectin and CXCL1 to the venular surface. Before surgery, some mice were injected intravenously with blocking mAbs to P-selectin (P-sel), β₂ integrins (β₂), CXCL1, or CXCR2, or with PTx. Basal neutrophil rolling flux fraction (E) and rolling velocity (F) were measured in dermal venules of ear in the absence of trauma. The data represent the mean ± SEM from 15 to 20 venules from 4 to 5 mice in each group. *P < .05, **P < .01.

This Feature Is Available To Subscribers Only