Figure 2
Figure 2. RXR loss induces growth differentiation and apoptosis of PML/RARA-transformed cells. (A) Generation of RXRAf/f RXRBf/f RXRG−/− PML/RARA FLT3-ITD Cre-ERT2 leukemia in mouse model. GFP-sorted spleen cells from leukemic mice were treated with 100 nM 4-OHT or 1 µM RA in culture and analyzed for cell cycle (B), PML/RARA expression (top bar, 10 µm) (C-D), and differentiation (E). RXRAf/f RXRBf/f RXRG−/− MLL/ENL Cre-ERT2 leukemia are shown as negative control (bar, 10 µm; MGG stain). IP, intraperitoneal; PI, propidium iodide.

RXR loss induces growth differentiation and apoptosis of PML/RARA-transformed cells. (A) Generation of RXRAf/f RXRBf/f RXRG−/− PML/RARA FLT3-ITD Cre-ERT2 leukemia in mouse model. GFP-sorted spleen cells from leukemic mice were treated with 100 nM 4-OHT or 1 µM RA in culture and analyzed for cell cycle (B), PML/RARA expression (top bar, 10 µm) (C-D), and differentiation (E). RXRAf/f RXRBf/f RXRG−/− MLL/ENL Cre-ERT2 leukemia are shown as negative control (bar, 10 µm; MGG stain). IP, intraperitoneal; PI, propidium iodide.

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