Cell cycle and apoptosis analyses of BM preleukemic cells expressing Cbfβ-SMMHC and Nras^G12D. (A-B) BM cells from mice treated as in Figure 1 were stained with 7-AAD and Annexin V and analyzed by flow cytometry: (A) the gating of each compartment is shown; (B) quantification of the ratio of apoptosis levels for LSK^– (left) and LSK⁺ (right) cells of each group normalized to control group (n = 5-11, *P < .05, **P < .01). (C-D) Flow cytometry analysis of cell-cycle status using Ki67 and Hoechst staining: (C) gating of BM progenitor for LKS⁺ and LSK^– compartments; (D) quantification (percentage) of cells in G₀ (white), G₁ (dotted), or S/G₂/M (gray) phase for each group (n = 4-8, *P < .05, **P < .01).