Characterization of leukemia in mice expressing Cbfβ-SMMHC and NrasG12D. (A) Kaplan-Meier curve of leukemia-free survival in Nras (dash), CM (solid), and Nras/CM (dotted) mice after Cre activation (time 0). (B) Kaplan-Meier survival curve of recipients transplanted with leukemic cells from CM (solid) and Nras/CM (dotted) mice. (C) Representative FACS analysis of peripheral blood cells from wild-type control and moribund CM or Nras/CM mice, showing progenitor cell marker c-kit (x-axis) and lineage markers (y-axis). (D) Representative hematoxylin and eosin (H&E)–stained histology sections of BM, spleen, and liver from wild-type control, moribund CM, Nras/CM mice, and Nras/CM secondary leukemic mice. LK, leukemia. The scale bars in the BM and spleen represent 50 μm; in the liver 100 μm.
