CD4⁺ iNKT cells promote a Th2-biased cytokine response in vivo. (A) mIFN-γ and mIL-5 staining of live Thy1.1⁺FoxP3^–CD4⁺ T cells. Gates were set on isotype controls. Shown are representative dot plots from 1 of 2 independent experiments. (B) Serum levels of cytokines in the presence of Tcon or Tcon + CD4⁺ iNKT cells. Shown are 3 animals per group from 1 of 3 independent experiments. Error bars indicate standard error of the mean. mTNF-α, murine tumor necrosis factor α. *P ≤ .05; **P ≤ .01.