Infusion of α-GpIbα induces dose-dependent platelet depletion. PCs were determined in citrated whole blood by flow cytometry. (A) Log scale scatter plots of CD61-positive events in naive mice infused with 2 μg/g α-GpIbα, 0.4 μg/g α-GpIbα, or control IgG. PCs depicted as scatter dot plots with the median 14 and 46 hours after IV administration of 2 μg/g (open dots), 0.4 μg/g α-GpIbα (half-open dots), or control IgG (closed dots) in naive mice (B) and in Klebsiella-infected mice (C). (D) PF4 was measured in naive or in infected control and platelet-depleted mouse plasma. Data are expressed as scatter dot plots with the median. n = 4 (naive) mice and n = 8 (infected) mice per group. *P < .05 and ***P < .0005 vs control IgG; ###P < .0005 vs 0.4 μg/g α-GpIbα.