Effect of chromatin reconstitution on purified DNA-dependent activation of coagulation. Chromatin was reconstituted using DNA and histones purified from normal human neutrophils. (A) Detection of nucleosomes in samples following reconstitution; positive and negative controls are provided with the ELISA kit. (B) TG performed in recalcified PFP in the presence or absence of 30 μg/mL kaolin (positive control), 50 μg/mL dialyzed hnDNA, dialyzed histones, or reconstituted chromatin. (C) Effect of dialyzed hnDNA, histones, and reconstituted chromatin on FXIa generation in the presence of physiologic concentrations of FXII, FXI, and HMWK in buffer. (D) Effect of dialyzed hnDNA, histones, and reconstituted chromatin on 10 nM α-thrombin-dependent activation of FXI (30 nM) in buffer in the presence of physiologic concentrations of HMWK. hnDNA, purified hnDNA. Panel B is representative of 3 independent experiments. Mean ± standard deviation of 3 independent experiments in panels A, C, and D.