Constitutive lentiviral Ifnγr1 overexpression in Ifnγr1^−/−macrophages. (A) Schematic representation of the lentiviral vector Lv.SFFV.Ifnγr1 expressing the Ifnγr1 cDNA by an SFFV promoter coupled to a GFP reporter via an internal ribosomal entry site (IRES). (B) GFP expression in Ifnγr1^−/− macrophages (left) without and (middle) with transduction using the Lv.SFFV.Ifnγr1 vector. (Right) Histogram depicting IFNγR1 (CD119) expression in Ifnγr1^−/−, WT, and Lv.SFFV.Ifnγr1-transduced macrophages compared with an isotype control. (C) WT, Ifnγr1^−/−, and Lv.SFFV.Ifnγr1 macrophages presenting characteristic morphology in representative cytospins. (D) Characteristic macrophage surface marker expression of CD11b, CD200R, and F4/80 at similar levels on WT, Ifnγr1^−/−, and Lv.SFFV.Ifnγr1-transduced macrophages compared with unstained samples. cPPT, central polypurine tract; FSC, forward scatter; GA, truncated GAG (group-specific antigen) sequence; LTR, long terminal repeat; ψ, packaging signal; R, redundant region; RRE, Rev-responsive element; SA, splice acceptor site; SD, splice donor site; U3, U3 region; U5, U5 region; wPRE, Woodchuck hepatitis virus posttranscriptional response element; WT, wild type.