Engraftment of lentiviral Ifnγr1 overexpressing cells rescues Ifnγr1^−/−mice from lethal pulmonary BCG infection. (A) Schematic representation of the experimental outline. Ifnγr1^−/− mice were lethally irradiated and transplanted with lineage-negative Ifnγr1^−/− cells transduced with Lv.SFFV.Ifnγr1 or Lv.MSP.Ifnγr1. After hematopoietic reconstitution, DsRed-labeled BCG was instilled into the lungs of the transplanted mice. Final analysis was performed 9 weeks after infection. (B) VCNs integrated into cells in the bone marrow (BM) and lungs (L) of transplanted and infected mice. Equal-size symbols represent VCNs of the same mouse in BM and L (n ≥ 3). (C) Kaplan-Meier curve depicting the survival of mice after BCG infection in 2 independent experiments (positive control [ctrl], 6 mice receiving WT cells; negative control, 13 mice receiving Lv.GFP-transduced Ifnγr1^−/− cells, 7 mice receiving Lv.SFFV.Ifnγr1, and 6 mice receiving Lv.MSP.Ifnγr1. (D) IFN-γ serum levels of mice at 2, 4, 6, and 8 weeks after infection. Mean and SEM are shown for 3 independent experiments (positive control, 9 mice that received WT cells; negative control, 9 mice that received Lv.GFP-transduced Ifnγr1^−/− cells and 7 nontransplanted Ifnγr1^−/− mice that received Lv.SFFV.Ifnγr1 and Lv.MSP.Ifnγr1.