Figure 4.
Warfarin uncouples full KO to KH2reduction in cells expressing warfarin-resistant mutants. (A) Western analysis with anti-VKORC1 antibody was performed on r-fIX BHK cells expressing r-VKORC1 variants or untransfected cells (C). (B) Specific activities were determined by assaying the cells as in Figure 3B and normalizing activity to protein expression. (C-D) Warfarin sensitivity was monitored as in Figure 3C. (E) Warfarin inhibition of carboxylation dependent on the Y139F K to KH2 reaction was tested as in Figure 3D.
