Pml NB disruption improves induction of APL. (A) Kaplan-Meier curve showing cumulative incidence of APL in Pml^WT (n = 249), Pml^C62A/C65A (n = 251), Pml^WT+p50-RARα (n = 252), and Pml^C62A/C65A+p50-RARα (n = 251). (Bi-ii) Representative pictures of blood smear of moribund Pml^C62A/C65A+p50-RARα mice showing hyperleukocytosis (May-Grünwald Giemsa staining; original magnification ×20 (i) and ×100 (ii)). (Biii) Representative spleens of Pml^WT healthy control and Pml^C62A/C65A+p50-RARα leukemic mice. (C) Kaplan-Meier survival curve of mouse primary recipients transplanted with Pml^WT+p50-RARα (n = 14) and Pml^C62A/C65A+p50-RARα (n = 8) leukemic BM cells, from 3 independent leukemias each. Mouse recipients transplanted with PML-RARα leukemic BM cells (n = 13) from 3 independent leukemic samples. This graph represents pooled data from 2 independent experiments. (A,C) Log-rank tests were used to compare survival curves.