Hepcidin blocks iron export from human RBCs without degrading Fpn. Human PRBCs from 4 different donors were treated with hepcidin or PR73 for 24 hours at 37°C. (A) Iron export from PRBCs was assessed by measuring NTBI in the medium. For the statistical analysis, the 2-tailed 1-sample Student t test was used with 100% as the comparison. (B) Western blotting of Fpn and actin levels in PBRCs.