HSP110 expression has an impact on NF-κB signaling. (A) Immunoblot analysis of HSP110, p-IκBα, p-p65, IκBα, and p65 at 48 hours after transfection with an siRNA targeting HSP110 or a control siRNA. β-actin served as a loading control. (B) p-IκB band intensity from immunoblots shown in (A) and determined from all cell lines (except U2932) relative to the loading control, data are presented as the mean ± standard deviation (n = 5; not U2932; P < .01). (C) Immunoblot analysis of HSP110, p65, and p50 content in the nucleus and cytosol of OCI-Ly3 and SU-DHL2 48 hours after transfection with an siRNA targeting HSP110 or a control siRNA. β-actin served as a loading control (n = 3). (D) Immunoblot analysis of HSP110, p-IkB, and IκB in SU-DHL2 cells 48 hours after transfection with plasmids coding for HSP110-GFP (n = 3). HSC70 served as a loading control. (E) Kinetics of cell survival determined by annexin-V/7AAD staining on HBL1 stably expressing either shRNA HSP110, shRNA control plus IKK2-EE, or shRNA HSP110 plus IKK2-EE, data are presented as the mean ± standard deviation (n = 3). **P < .01; ***P < .001.