Mediator production and release in platelets is stimulus dependent and centered. (A) Concentration (pmol/mg_protein) of 12-HETE, arachidonic acid and PI 18:0-20:4. (B) Bar graphs of various mediator precursors during different stimuli. (C) Analyzed mediators in platelets and their corresponding secretome level. Bar graphs with black-framed colors display mediators in platelets; bars with red-framed colors indicate mediators in the corresponding secretome. (D) Measured lipid regulation during platelet activation. The absolute quantities are reported in pmol/mg_protein. Data were combined from 3 independent experiments, displayed as mean values, and the error is represented as standard deviation of the mean. #The lipid class of free FAs (FFA); ##Eicosanoids; §Docosanoids; §§Octadecanoids. N.D., not detectable.