Recruitment of neutrophils and enhancement of venous thrombosis. The activated endothelium expresses P-selectin, ICAM-1, and CXCL1 attached to proteoglycan. Neutrophils are first captured by the activated endothelium by an interaction between P-selectin and PSGL-1, followed by an interaction between CXCL1 and its receptor, CXCR2. Intracellular signaling induced by engagement of PSGL-1 and CXCR2 leads to conformational activation of the αLβ2 (LFA-1) integrin that interacts with ICAM-1 and mediates firm adhesion. NET formation is triggered by signals downstream of PSGL-1 and CXCR2. It is possible that signaling downstream of αLβ2 (LFA-1) also contributes to NET formation. Professional illustration by Somersault18:24.