Thrombin-stimulated platelet MV release and PS exposure are shear dependent and require integrin α_IIbβ_3. (A) MV release in resting wild-type (WT) and β₃ knockout (KO) mouse platelets under different shear rates as analyzed by flow cytometry. (B) MV release in 0.05 U/mL of thrombin-stimulated WT and β₃ KO mouse platelets under different shear rates. (C) PS exposure on the surface of resting WT and β₃ KO mouse platelets under different shear rates as indicated by annexin V binding. (D) PS exposure on the surface of 0.05 U/mL of thrombin-stimulated WT and β₃ KO mouse platelets under different shear rates as indicated by annexin V binding. (E) MV release from WT and β₃ KO mouse platelets stimulated with various doses of thrombin (0.0125, 0.025, and 0.05 U/mL) with or without shear (shear rate 3000 s⁻¹). (F) PS exposure on the surfaces of WT and β₃ KO mouse platelets stimulated with various doses of thrombin (0.0125, 0.025, and 0.05 U/mL) with or without flow shear. Data are presented as mean ± standard error of the mean (n = 3-4). **P < .01, ***P < .001, ****P < .0001.