The effect of mP6 on fibrin generation and platelet thrombus formation in vivo using laser-induced mouse arteriolar thrombosis model. Intravital microscopy was used to monitor fibrin generation and platelet thrombi in vivo after laser-induced cremaster arteriole wall injury in wild-type mice treated with or without selective outside-in signaling inhibitor mP6 (10 µmol/kg) or scrambled control peptide (mP6Scr; 10 µmol/kg). The median integrated (Int.) fluorescence (FL) signals of fibrin (A) and platelets (B) from 28 thrombi in mP6-treated mice, 26 thrombi in mP6Scr-treated mice, or 27 thrombi in untreated mice are shown as a function of time. The median of the total FL detected over time for fibrin (C) or platelets (D) is shown, calculated by integrating the area under the curve. (E) Representative images of fibrin generation (green) and platelet thrombi formation (red) and merged images. ****P < .0001.