Schematic representation of the method used to evaluate T-cell polyfunctionality. (A-B) Schematic representations of CAR construct configuration and treatment protocol. (C-D) Product T-cell polyfunctionality was assessed by using enzyme-linked immunosorbent assay (ELISA) detection of proteins from each single-cell chamber after T-cell stimulation. (E-F) Polyfunctionality was measured through a PSI, spanning a prespecified panel of 32 key immunologically relevant molecules across major categories: homeostatic/proliferative, inflammatory, chemotactic, regulatory, and immune effector. cyt, cytokine; MIP-1α, macrophage inflammatory protein 1α; scFv, single-chain variable fragment.