TgRhoA mice have increased TFHcompartments. (A) Splenic CD4+ cell expression of CXCR5 and PD-1 in CD4 cells from 10-week-old WT vs tgRhoA littermates. (B) ICOS expression in CD4+CXCR5+PD-1+ from WT and tgRhoA littermates. Naive CD4 T cells in both plots are from WT mice. (C) Percentage of CD4+ cells that are CXCR5+PD-1+ (P = .0021). (D) ICOS mean fluorescence intensity among CD4+CXCR5+PD-1+ from both genotypes (P = .0472). (E-H) The same approaches in (A-D) applied to 30-week -old mice. P = .0093 for (G). P = .0397 for (H). (I) ELISA of serum IgG (top, P = NS) and double-stranded DNA (bottom, P = .0273) from 30-week-old mice. (J) Immunofluorescence of representative glomeruli from WT or tgRhoA mice. Mouse IgG, green (Alexa 488); DAPI, blue. Scale bar, 50 μM. (K) IgG deposition from WT or tgRhoA littermates was quantified from at least 7 different sections per mouse, and the mean IgG deposition per section is shown for WT and tgRhoA (4.26 vs 9.61; P = .0206). All P values from t test with Welch’s correction.
