Expression of G6b isoforms in humanized mouse model. (A) WT, G6b^+/hu, G6b^hu/hu, human and G6b^−/− washed platelet lysates (4 × 10⁸/mL), were resolved by SDS-PAGE and custom antibodies used to detect mouse G6b-B (mG6b-B), human G6b-B (hG6b-B ), and hG6b-A. Expression was quantified and normalized to (i,ii) WT or (iii,iv) human lysates, to show relevant expression levels in the mouse models. (B) Quantification of glycosylated and unglycosylated human G6b-A and -B, data normalized to show percentage of total expression. (C) hG6b-B was immunoprecipitated (IP) from basal and collagen-activated G6b^hu/hu and human washed platelet lysates (4 × 10⁸/mL). Co-IP of Shp1 and Shp2 was investigated by (i) SDS-PAGE before (ii) quantification and normalization to hG6b-B levels. Quantification was completed using Licor Odyssey system. All data represented as mean ± SEM (n = 3-4). ***P < .001.