Cytological and ultrastructural characteristics of bone marrow granulocytic cells. (A-B) Cytology analysis after May-Grünwald Giemsa staining. Pictures represent granulocytic precursors (a-f) and neutrophils (g-i)) in different individuals P3, P10, P19, P20, and P23. (Original magnification ×100). (C) Comparative cytological bone marrow analysis of 16 individuals with SRP54 mutations and 16 patients with ELANE mutations Significant difference was observed between SRP54 and ELANE patients using nonparametric Kruskal-Wallis test (P = .02). (D-E) Analysis by TEM of the ultrastructural aspect of bone marrow cells from the granulocytic lineage in patient P1. Enlarged ER was observed around the nucleus (N) and thorough the cytoplasm (Cyt) in promyelocytes (D). The black arrow points to nascent autophagosome. Right panels (E) show pre-apoptotic neutrophil granulocytes, a major characteristic of the sample.