Deletion of Zmiz1 inhibits thymocyte recovery after sublethal irradiation. (A-B) Flow cytometric profiles of CD4/CD8 thymic subsets (A) and absolute number of thymocytes (B) on the indicated days after sublethal irradiation (450 rad). (C-G) Representative images of thymuses (C); absolute numbers of total thymocytes and DN thymic subsets (D); representative flow cytometry profiles of DN subsets (E); representative flow cytometry profiles of CD4/CD8 thymic subsets (F); and absolute numbers of CD4/CD8 thymic subsets (G) of LckCre and Zmiz1^ΔLckCre (ΔLckCre) mice at 6 days after sublethal irradiation. (H-I) Representative flow cytometric plots of DN3b cells (H) and scatterplots of DN3a, DN3b, and DN4 (I) analyzed for 5-ethynyl-2'-deoxyuridine (EdU) vs DAPI in LckCre and ΔLckCre mice. Mice were injected with EdU at 3 hours prior to analysis. (J) qPCR showing Zmiz1 and Myc transcripts in sorted DN3 cells on day 6 after sublethal irradiation relative to mock irradiation (N = 3). Mice were irradiated at 5 to 8 weeks of age. *P < .05; **P < .01; ****P < .0001. Two sample Student t test. DN3a = lineage⁻CD44⁻CD25⁺CD28⁻; DN3b = lineage⁻CD44⁻CD25⁺CD28⁺; DN4 = lineage⁻CD44⁻CD25⁻CD28⁺icTCRβ⁺; ISP = CD8⁺TCRβ⁻. FC, fold change; FDR, false discovery rate; NES, normalized enrichment score; RNA-Seq, RNA sequencing.