Absence of Ezh2 leads to cell-extrinsically mediated anemia. (A) Representative FACS plots showing distinct block in E12.5 FL erythropoiesis in Tie2-Ezh2-KO but not Vav-Ezh2-KO embryos. Shown are the mean values with SEM for the frequencies of the indicated population across all experiments. (B) Absolute numbers of E12.5 FL erythroid progenitor populations (Tie2-Ezh2-WT, n = 26, 5 independent experiments; Tie2-Ezh2-KO, n = 7, 4 independent experiments; Vav-Ezh2-WT, n = 6, 2 independent experiments; Vav-Ezh2-KO, n = 5, 2 independent experiments). Two-way analysis of variance was used to assess statistical significance. Error bars represent SEM. (C) Peripheral Gata1-EGFP⁻ erythroid (Ter119⁺) reconstitution kinetics in CD45.1 Gata1-EGFP recipient mice transplanted with 500 000 CD45.2 Tie2-Ezh2-WT (n = 7), Vav-Ezh2-WT (n = 2), Tie2-Ezh2-KO (n = 12) or Vav-Ezh2-KO (n = 3) total E12.5 FL cells; Vav-Ezh2-WT (n = 5) or Vav-Ezh2-KO (n = 7) E18.5 FL cells, together with 1 000 000 CD45.1 Gata1-EGFP competitor BM cells. Data are pooled from 1 (Vav-iCre; E12.5) or 2 (Tie2-Cre; E12.5 and Vav-iCre; E18.5) independent experiments. Two-tailed Student t tests were used to assess statistical significance. Error bars represent SEM.