Ring1B binds to the Glis2 promoter region and is associated with histone modification of Glis2. (A) Western blotting showing levels of total H2AUb1 in MOZ-TIF2 leukemic cells cultured for 48 hours in the presence or absence of 4-OHT. (B) Diagram illustrating the Glis2 locus. Each of the regions amplified by the indicated primer sets are indicated by black rectangles. (C-F) Chromatin immunoprecipitation analyses of the Glis2 locus in Ring1A- or Ring1A/B-knockout MOZ-TIF2 leukemic cells using anti-Ring1B, anti-H2AUb1, anti-H3K4 me3, and anti-H3K27 me3 antibodies. The Myf5 and Ink4a/Arf promoter regions were used as controls. Error bars represent SD (n = 3).