Overexpression of Glis2 induces cell differentiation and suppresses leukemic transformation of cells expressing MOZ-TIF2. (A) Western blot analysis of MOZ-TIF2–expressing leukemic cells transduced with Glis2 or empty vector. MOZ-TIF2 leukemic cells were transfected with GCDNsam-IRES/NGFR-Glis2 or empty vector and then stained with anti-NGFR-APC. NGFR⁺ cells were magnetically labeled with anti-APC Microbeads and isolated by MACS. (B) Quantitative RT-PCR analysis of Glis2 expression. Error bars represent SD (n = 4). (C) Representative colony morphology and May-Grünwald Giemsa staining of MOZ-TIF2 leukemic cells transduced with Glis2 or empty vector. Scale bar, 50 μm. (D) Serial replating experiments of MOZ-TIF2 leukemic cells transduced with Glis2 or empty vector. Error bars represent SD (n = 3). (E) Flow cytometry analysis of MOZ-TIF2 leukemic cells transduced with Glis2 or empty vector after staining for F4/80 or CD14. Error bars represent SD (n = 3). (F) Kaplan-Meier survival curve analysis of mice that received MOZ-TIF2 leukemic cells transduced with Glis2 or empty vector.