Activation of NADPH oxidase during efferocytosis in a CD11b-MyD88–dependent manner. PEMs from WT, CD11b^−/−, and CD44^−/− mice were either stimulated with (A) hANs or (B) zymosan, and ROS production measured as lucigenin-elicited chemiluminescence. (C) WT PEMs were stimulated with hANs or hANs opsonized with pooled human serum and ROS detected using lucigenin. (D) PEMs from WT and Myd88^−/− mice were stimulated with hANs. For each graph, total integrated responses measured as relative light units/s (RLU) recorded over 1 hour are shown. Data from 1 of 3 experiments (duplicate wells) are shown as mean ± SD. Statistical differences between groups were calculated using 2-way ANOVA with Bonferroni posttest correction. *P < .05; ϕP < .01.