CGD macrophages have increased cross-presentation of AC-derived antigens. (A) WT and CGD PEMs were plated in Permanox chamber slides, cultured overnight, and the next day stimulated with hANs for an additional 18 hours. Cell-free supernatants were analyzed by 32-cytokine multiplex cytokine arrays or enzyme linked immunosorbent assays (transforming growth factor β [TGF-β] and prostaglandin E2 [PGE-2]). Data from 1 of 2 experiments using 3 mice per genotype are shown as mean ± SD. Statistical differences were determined by 1-way ANOVA with the Tukey postcorrection test. **P < .01. (B-C) WT and CGD PEMs were incubated for 72 hours with either varying numbers of OVA-loaded ATs or soluble OVA in the presence of CFSE-labeled CD8 T cells derived from transgenic OT1 mice. At the end of 72 hours, proliferation of CD8 OT1 T cells was measured by flow cytometry as CFSE dilution, and the percentage of proliferated T cells determined for each stimulation condition and agonist concentration. Data (mean ± SD) from 1 of 3 representative experiments are shown. **P < .01 using the Student t test.