Regional heterogeneity of cAlb transport properties. (A) A representative image of a thrombus generated using a laser injury model in mouse cremaster arterioles (3 minutes postinjury). Thrombi were monitored for platelet deposition (blue), P-selectin exposure (red), and cAlb (not shown) for 0 to 3 minutes post-injury. (B) The growth of platelet (blue) and P-selectin(+) (red) areas were monitored over 3 minutes. The areas were measured by gating the images on either CD41(+) or P-selectin(+) pixels and then averaged across 20 injuries from 5 WT mice. (C) Thrombus regions were defined as CD41(+)/P-selectin(+) and CD41(+)/P-selectin(−) for cAlb analysis. Pulses of 405-nm light were used to activate the cAlb infused prior to injury, and the normalized decay curves for the P-selectin(+) (red) and P-selectin(−) (blue) regions were averaged over each pulse for 0 to 3 minutes postinjury (±SD).