Dynamics of thrombus regional porosity. Albumin labeled with Alexa Fluor 488 was infused into the mouse prior to injury and the fluorescence was monitored within the lumen, LTR, and shell of the developing thrombi. (A) A representative image of platelets (blue) and LTR (white outline) 120 seconds postinjury. (B) For the same thrombus, a pseudo-colored image of the thrombus colored to represent the porosity based on the normalized fluorescence intensity. (C) Average porosity of the shell (blue) and LTR (cyan) of 8 individual injuries over 240 seconds postinjury.