Thrombin activity within the LTR. (A) A representative image of thrombin activity (mThS-Ab; green, binary mode) within a thrombus 20 minutes after injury also showing platelet deposition (blue) and core formation (red). Overlay of 3 channels is white. (B) The change in the mean mThS-Ab fluorescence was monitored within the LTRs (cyan) and shell (blue) during the initial 3 minutes after injury and again at 20 minutes (±SEM).