The role of contact-dependent Sema4D signaling in LTR and core formation. (A) Representative images of a thrombus generated in Sema4D^−/− and WT mice cremaster muscle arterioles showing platelets (blue) and P-selectin (red) at 3 minutes postinjury. (B) Quantification of the average P-selectin(+) area within Sema4D^−/− and WT thrombi at 3 and 20 minutes postinjury (n = 13 for both Sema4D^−/− and WT; ^+/− SD) showing a lag in P-selectin exposure in the knockout. (C) For each representative thrombus, the corresponding cAlb half-life heat map is shown with the LTR highlighted (cyan). (D) The cAlb decay curves of the LTR of wild-type (black) and Sema4D^−/− (gray) thrombi (WT, n = 10; Sema4D^−/−, n = 14; ±SD).