American Society of Hematology

Figure 1.

$Figure 1. Histological appearance of FL. (A) Hematoxylin-and-eosin staining of an FL grade 1 (original magnification ×40). Note the numerous follicles of the FL that lost the typical dark-zone/light-zone demarcation and are surrounded by thin mantle zones. (B) Ki-67 staining of an FL, revealing the typical low fraction of proliferating cells in an FL follicle (original magnification ×100). (C) BCL2 staining of an FL, showing strong BCL2 staining of the lymphoma cells (original magnification ×100). The center of the FL follicle is marked with an asterisk (*); the thin mantle zone surrounding the follicle is marked with a pound symbol (#). (D) BCL2 staining of a reactive lymph node with BCL2+ mantle-zone B cells and BCL2− GC B cells (original magnification ×100). For the BCL2 staining, a mouse anti-human monoclonal antibody (clone 124; DAKO Agilent, Hamburg, Germany) was used; for the Ki-67 staining, a mouse anti-human monoclonal antibody (clone MIB-1; DAKO). Stainings were kindly provided by S. Hartmann and M.-L. Hansmann (Senckenberg Center for Pathology, University of Frankfurt/Main, Frankfurt, Germany).$

Histological appearance of FL. (A) Hematoxylin-and-eosin staining of an FL grade 1 (original magnification ×40). Note the numerous follicles of the FL that lost the typical dark-zone/light-zone demarcation and are surrounded by thin mantle zones. (B) Ki-67 staining of an FL, revealing the typical low fraction of proliferating cells in an FL follicle (original magnification ×100). (C) BCL2 staining of an FL, showing strong BCL2 staining of the lymphoma cells (original magnification ×100). The center of the FL follicle is marked with an asterisk (*); the thin mantle zone surrounding the follicle is marked with a pound symbol (#). (D) BCL2 staining of a reactive lymph node with BCL2⁺ mantle-zone B cells and BCL2⁻ GC B cells (original magnification ×100). For the BCL2 staining, a mouse anti-human monoclonal antibody (clone 124; DAKO Agilent, Hamburg, Germany) was used; for the Ki-67 staining, a mouse anti-human monoclonal antibody (clone MIB-1; DAKO). Stainings were kindly provided by S. Hartmann and M.-L. Hansmann (Senckenberg Center for Pathology, University of Frankfurt/Main, Frankfurt, Germany).

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