Loss of β3 integrin tyrosine phosphorylation attenuates fibrin formation following vascular injury in vivo. (A-C) Total fibrin accumulation was monitored following laser-induced injury in mouse cremaster arterioles using a fluorescently tagged antibody that specifically recognizes mouse fibrin. The graphs in panels A and B show total fibrin area (A, mean ± SEM; B, median) over time. Panel C shows peak fibrin area for WT and diYF mice. The horizontal line and error bars indicate median and interquartile range. (D-F) Platelet-associated fibrin was defined as the area that was positive for both fibrin and CD41 fluorescence. The graphs in panels D and E show platelet-associated fibrin area (D, mean ± SEM; E, median) over time. Panel F shows peak platelet-associated fibrin area for WT and diYF mice. For WT, n = 30 thrombi from 6 mice and for diYF n = 19 thrombi from 4 mice. Statistics were calculated using the Mann-Whitney test for nonparametric data.