HTS analysis of TRB repertoire in CD4⁺T_reg, CD4⁺T_conv, and CD8⁺T-cell subsets. (A) Number of unique reads identified in each patient for T_reg, T_conv, and CD8⁺ T cells and (B) Shannon entropy index calculated among unique reads for T_reg, T_conv, and CD8⁺ T cells. (A-B) *P < .05; **P < .01. (C) Representative heat maps depicting Vβ and Jβ gene pairing in unique sequences from (left) CD4⁺ T_conv, (middle) CD4⁺ T_reg, and (right) CD8⁺ T cells from controls and patient 1. (D) Hydrophobicity analysis of amino acid residues at positions 6 and 7 of the TRB-CDR3 in (left) CD4⁺ T_conv, (middle) CD4⁺ T_reg, and (right) CD8⁺ T cells from CD3G-mutated patients (n = 6) compared with controls (n = 8). *P < 10⁻⁴.