Hemostatic plug formation in wt and HB mice with FV-810^SYA. Quantitative analysis of platelet (A) and fibrin accumulation (B) over time after laser injury to cremasteric arterioles in wt mice (n = 5; 23 thrombi), and HB mice treated with FV-810^SYA (n = 5; 22 thrombi; 600 µg/kg). The kinetics of platelet and fibrin accumulation were examined by monitoring the fluorescent intensity at the site of the injury using an Alexa₅₅₅-labeled rat anti- CD41 F_(ab)2 and Alexa₄₈₈-labeled anti-fibrin antibodies. Median fluorescence intensity (MFI) for platelets (A) and fibrin (B) fluorescence are plotted vs time. For both platelets and fibrin accumulation, there is no statistically significant difference (P > .1) in MFI (evaluated at 30-second intervals) between wt mice and HB + FV-810^SYA by the Wilcoxon rank-sum test.