Effect of integrilin on distribution and accumulation of fibrin. (A) Normalized fluorescence intensity of, Alexa₄₈₈-FV-810^SYA (green), Alexa₆₄₇-FXa_i (cyan), platelets (red) and fibrin (blue) over volume from multiple thrombi (n = 3-7 mice; 14-27 thrombi per group) generated at approximately 4 minutes after injury in HB or Par 4^−/− mice infused with labeled-FV-810^SYA FVa (600 µg/kg), Alexa₆₄₇-FXa_i (80µg/kg), antibodies against platelets, or fibrin with or without integrilin. Normalized fluorescence intensity was not acquired for FV-810^SYA and FXa_i for Par4^−/− mice in the presence of integrilin (NA). NA, not acquired. (B) Kinetics of fibrin accumulation over time in Par 4^−/− mice (n = 3 mice; 15 thrombi) or HB mice (n = 5 mice; 22 thrombi) infused with labeled Alexa₄₈₈-FV-810^SYA (600 µg/kg) in the presence or absence of integrilin (10 mg/kg). (C) Confocal imaging of labeled platelets, Alexa₄₈₈-FV-810^SYA, and labeled fibrin 240 seconds after laser injury, merged or presented individually; step size = 1 µm in z-plane; total section 30 µm. (C) Without integrilin (top); with integrilin (bottom). Platelet accumulation was monitored using Alexa₅₅₅-labeled rat anti-CD41 F_(ab)2 antibody and fibrin was detected with Alexa₆₄₇-labeled anti-fibrin antibody. (C) Bar represents 30 μm.