RUNX1 expression correlates with the induction of an endothelial-associated cell adhesion and migration program in the HE. (A) Principal component analysis of RNA-seq expression data from all biological replicates of Runx1^wt and Runx1^ko HE populations (n = 4). (B) Heat map depiction of all differentially expressed genes between Runx1^wt and Runx1^ko HE. A total of 387 genes (in red) are positively correlated with RUNX1 expression and 235 genes (in blue) are negatively correlated. (C) RPKM-mapped reads expression values of RUNX1 exons in Runx1^wt HE. Total expression was based on exon 8 and RUNX1 distal expression on exons 1 and 2. *P < .001. (D) GSEA showing significantly enriched biological processes and signaling pathways in the gene set positively correlated with RUNX1 expression in HE.