Overview of immunotherapy target discovery approach by Hu et al, including cloning, expression, and screening for T-cell activity against candidate antigens. APC, antigen-presenting cell; IFNγ, interferon-γ; IL-2, interleukin-2; L, lucerifase; Min., minimal; R, reporter. See Figure 1 in the article by Hu et al that begins on page 1911.