CD4⁺ transcriptional profiles from patients with HL are separated from RLN on the basis of TGFβ-regulated genes. CD4⁺ T cells were isolated from lymph nodes of 4 different patients with HL, 3 patients with FL, and 5 patients with RLN. cRNA was hybridized to HG-U133A Affymetrix arrays. The RNA fingerprints of (A) TGFβ and (B) PD-1 were used to separate transcriptional profiles of HL from RLN. Left panel of A and B: Hierarchic cluster analysis using average linkage and correlation distance metric. Right panel of A and B: Result of PCA with the first 3 principal components is shown. RNA fingerprints of (C) TGFβ and (D) PD-1 were used to attempt to separate transcriptional profiles of FL from RLN. Again, hierarchic cluster analysis and PCA are shown.