The intersection of GATA1 and GATA2 binding site sets reveals GATA1-selective, GATA2-selective, and GATA switch binding sites. (A) Venn diagram showing the intersection of binding sites between the sets of GATA1 and GATA2 binding sites. A total of 43% of GATA1-bound sites and 30% of GATA2-bound sites are GATA “switch sites.” (B-C) Intersection of GATA1 and GATA2 ChIP-Seq binding sites using relaxed binding site identification parameters (P < .01) for one of the factors to allow for identification of a high confidence set of GATA1-selective or GATA2-selective binding sites. (D) Bar graph showing the expression profiles of 3321 GATA switch-bound genes. Data are depicted as relative average expression in GATA1-restored condition compared with the MIGR1 vector control condition. Two of the most strongly induced genes (Vwf and Thbs1) are indicated on the plot. Cpa3, Kit, and Gata2 are strongly repressed by GATA1 restoration and are also indicated on the plot. (E-F) GATA1 and GATA2 binding profiles at the Vwf and Kit loci as in Figure 2F through H. (G) Real-time quantitative PCR to confirm down-regulation of Cpa3 and Kit and the induction of Thbs1 and Vwf following GATA1 restoration in G1ME cells. PCRs were performed in triplicate from biologic duplicates 72 hours after infection. Bars represent the mean relative expression in GATA1-restored condition compared with the MIGR1 vector control condition; error bars represent SD.