Figure 7
IgM binding to red cells treated with exogenous PLA2. (A) Rat red cells were prelabeled with the bodipy FL11 PC probe (1 h at 4°C) by ethanol injection17 before overnight incubation with PLA2 at 4°C. Red cells were then analyzed by flow cytometry to assess probe hydrolysis and fluorescence (FL1) intensity increase. After cell treatment with PLA2, IgM was provided in the form of rat plasma (B) or carbonate wash (C), and IgM association to the red blood cells was monitored. Tinted patterns indicate untreated cells; –, cells treated with 65 U of PLA2; and —, cells treated with 130 U of PLA2.
