Rapid consumption of TAFI protein occurs with a lethal dose of E coli (LDEC), however treatment with the mAbTAFI/TM#16 slows consumption; TAFI consumption is not observed in the 24-hour period following a sublethal dose of E coli (SLEC). (A) Plasma samples from a representative animal that received a LDEC reveal consumption of TAFI protein at 480 minutes after E coli infusion by Western blotting. When the LDEC treatment was administered concurrently with the mAbTAFI/TM#16, rapid consumption of TAFI was not observed. (B) A representative animal that received a SLEC was monitored for 24 hours after E coli treatment. TAFI protein was present in all plasma samples and no difference was observed in the absence or presence (data not shown) of mAbTAFI/TM#16 infusion.