Direct cytotoxicity induced by anti-CD20/CD74 HexAbs in NHL cell lines as determined by the MTS assay. (A) JeKo-1, Granta-519, Mino, and Raji (5 × 10⁴ cells per well in 48-well plate) cells were treated with indicated concentrations of Abs for 4 days. (B) Effect of monospecific 20-(20)-(20) on JeKo-1, Granta-519, and Mino cells; bispecific 20-(22)-(22) on JeKo-1 cells; and monospecific 74-(74)-(74) on Raji cells. (C) Dose-response curves showing partial inhibition of 20-(74)-(74) and 74-(20)-(20) in JeKo-1 cells by excess hA20 or hLL1 (10 μg/mL). Error bars represent (SD); n = 3.