Induction of apoptosis by anti-CD20/CD74 HexAbs. (A) JeKo-1, (B) Granta-519, and (C) Mino cells (2 × 105 cells per well in 6-well plate) were treated with 10nM of indicated Abs for 48 hours followed by annexin staining analysis. The 2 bispecific anti-CD20/CD74 HexAbs induced statistically significant apoptosis in all 3 cell lines compared with cells treated or not treated with parental Abs, alone or combined. (D) Annexin analysis on primary samples from patients with MCL treated with indicated Abs (10nM) for 24-48 hours. (E) Annexin analysis on primary samples from patients with CLL treated with indicated Abs (10nM) for 24-48 hours. (D-E), Data are shown as annexin+ PI− cells (early apoptosis). The 2 anti-CD20/CD74 HexAbs induced statistically significant early apoptosis in MCL and CLL compared with the untreated controls. One of the patient samples (CLL 216) did not respond to any treatment. Error bars represent SD (A-E); n = 3.
