Activity of HexAbs in human bloodex vivo. The effect of the indicated Abs on the growth of spiked JeKo-1 cells in whole blood from a healthy volunteer was determined after 48 hours. JeKo-1 cells were analyzed as CD19⁺ events in the monocyte gate. B cells were analyzed as CD19⁺ events in the lymphocyte gate. Error bars represent SD; n = 3. (A) 20-(74)-(74) and 74-(20)-(20) were tested at 10nM and 25nM in JeKo-1 (left panel) and normal B cells (right panel). (B) 20-(74)-(74) was tested at 0.1nM, 0.5nM, and 1nM in JeKo-1 cells (left panel) and normal B cells (right panel). (C) 74-(20)-(20) was tested at 0.1nM, 0.5nM, and 1nM in JeKo-1 cells (left panel) and normal B cells (right panel).